Ceva Biovac mycoplasma antigens

Mycoplasma are very small bacteria (between 0.3 and 0.8 μm in diameter) characterised by the absence of cell wall. Clinical signs vary depending on the species: respiratory symptoms for M. gallisepticum, infectious synovitis for M. synoviae, and immunosuppression in turkeys for M. meleagridis. ¹

The rapid plate agglutination (RPA) test is a technique based on the principle of agglutination of specific serum antibodies produced by infected poultry with the suspension of corresponding antigens, present in the Ceva Biovac reagents. Agglutination reveals the presence of circulating antibodies.

Advantages

Early antibody detection The RPA is the only test to detect IgM and IgG at the same time. It can thus be used to detect infection several days in advance of ELISA. 2, 3, 4	Increased flexibility The tests can be performed individually. The user can choose exactly how many tests to perform.
Time-efficient The test is easy to set up. The results are obtained in a few minutes.	Reduced costs As well as the low operating costs (1 bottle for 400 tests), the investment required to set up the tests is small.

_{(1) S. Baron, Medical Microbiology, 4th edition,1996(2) S. Collett, Monitoring Broiler Breeder Flocks for Mycoplasma gallisepticum Infection after Vaccination, thesis, 2004(3) S. Asgharzade, Detection of Mycoplasma gallisepticum in experimentally infected broiler chickens using Culture, SPA, ELISA, and PCR methods , Comparative Clinical Pathology, vol. 22, issue 6, p. 1051-1055, 2013(4) AFNOR Standard U47-012, 2009}

Ceva Biovac products

Reagents

• Mycoplasma gallisepticum (ref: AS9)
• Mycoplasma meleagridis (ref: AS11)
• Mycoplasma synoviae (ref: AS6)

Control sera

• Specific pathogen-free (SPF) chicken serum  (ref: AS7)
• Positive chicken Mycoplasma gallisepticum (ref: AS10)
• Positive chicken Mycoplasma meleagridis (ref: AS12)
• Positive chicken Mycoplasma synoviae (ref: AS8)

Instructions for use

Click below to download the instructions for use for the different rapid plate agglutination tests:

• Mycoplasma gallisepticum antigen
• Mycoplasma meleagridis antigen
• Mycoplasma synoviae antigen
• Specific pathogen-free (SPF) chicken serum
• Positive chicken Mycoplasma gallisepticum serum
• Positive turkey Mycoplasma meleagridis serum
• Positive chicken Mycoplasma synoviae serum

Reading the results

The results are easy to read. Blue-violet agglutinates indicate a positive result and nothing happens when the result is negative. An aid to reading the results can be downloaded here. It includes photos of positive and negative results.

Safety data sheets

• SDS Mycoplasma gallisepticum
• SDS Mycoplasma meleagridis
• SDS Mycoplasma synoviae

Also in avian section :

• Salmonella

• Reagents